Thesis On Isolation Of Bacteria

Thesis On Isolation Of Bacteria-72
The greatest challenge to the environmentalists is the eco friendly management of this waste and application of microorganisms in this context has got an age over other available technologies.

The greatest challenge to the environmentalists is the eco friendly management of this waste and application of microorganisms in this context has got an age over other available technologies.

The samples were weighed several times until a constant weight was achieved.

Samples moisture content was calculated employing the following formula provided by AWPA.100 Where, MC is moisture content, W is the original weight and w is the constant weight after oven drying.

Optimization of growth condition Three semi-solid media as NA (Nutrient Agar), BCDA (czapek dox agar medium (Basic)) and ACDA (Czapek Dox Agar medium (Acidic)) were used to optimize the cultural media of isolated bacteria.

The p H were adjusted to 5.2, 6.5, 7.2, 8.9 and 10.2 in NA medium; 2.2, 3.2, 4.0, 5.5 and 6.9 in BCDA medium; and 7.1, 7.6, 9.1, 10.06 and 12.10 in ACDA medium.

In this technique sample suspension was prepared by adding soil mixed with waste (1g) was added to 10ml of sterile water (the stock) and shaken vigorously for at least 1 minute. Sterile dilution blanks were marked sequentially starting from stock and10.

From each dilution tube 0.1ml of dilution fluid was transferred into Nutrient Agar culture media and incubated at 37°C for 24hours.

Extent of microbial diversity in nature is still largely unknown, thus there might be many more useful products yet to be identified from soil microorganisms.

In soil 80 to 99% of microorganisms remain unidentified whereas these biological communities are known to play a dominant role in maintaining a sustainable biosphere.

Determination of moisture content (%) and p H of waste samples Freshly collected samples were kept in filter paper and the initial weight was recorded.

After that these samples were kept inside a hot air incubator at 110 °C.

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