Stem Cell Thesis

Due to its ability to allow complete cell removal, the smaller void size scaffold was used to develop a method for long-term PSC culture.This, however, resulted in gradual cell differentiation, suggesting that a mixed-topography was more suited for PSC growth.

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Neural stem cells (NSCs) are characterized by the ability of self‑renewal and capacity to proliferate and produce new nervous tissue.

NSCs are capable of differentiating to three lineages of neural cells, including neurons, oligodendrocytes and astrocytes.

This was attributed to a marked decrease in the nucleation of actin, providing a correlative link to the enhanced differentiation possible after 3D priming.

Additionally, there were significant differences in the presence of cell-matrix adhesions as well as evidence of reorganisation of the nuclear lamina.

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Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.Furthermore, the expression of neurotrophin 3 (NT3), brain‑derived neurotrophic factor (BDNF), glial cell‑derived neurotrophic factor (GDNF) and transforming growth factor (TGF)β1 was also investigated in ts NSCs and r NSCs.The expression of all of the aforementioned proteins was detected using immunofluorescence methods.However, their use for such purposes is hampered for a number of reasons.Both the reprogramming of somatic cells into PSCs and their differentiation for use in research and the clinic are extremely low yielding.Nestin was used as a marker to identify the cultured NSCs.Neuronal nuclei protein and glial fibrillary acidic protein (GFAP) were utilized to demonstrate the differentiation of NSCs towards neurons and astrocytes, respectively, in vitro.Furthermore, the expression of NT3 in the ts NSCs was higher compared with r NSCs (P Neural stem cells (NSCs) are a type of stem cell that possess self-renewal, self-replication and multi-differentiation properties.Under certain conditions, NSCs may be induced to differentiate into neurons, astrocytes and oligodendrocytes (1–3).Cardiovascular diseases (CVD) are the leading cause of death in developed societies.Vascular stem cells (VSCs) have been proposed as a cell population with regenerative potential for CVD.

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