Daniel Wagenaar Thesis

Daniel Wagenaar Thesis-25
I designed and built hardware to deliver electrical stimuli in arbitrary patterns, developed software to remove stimulation artifacts from recordings, and studied the efficacy of several voltage-defined and current-defined stimulus waveforms. Thus, stimuli can be made dependent on a culture's activity with only 15 ms lag-time.

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Activity patterns varied widely in terms of the frequency, intensity, duration, and degree of temporal clustering of bursts.

During the 2nd week in vitro, bursts in many dense cultures clustered into well-defined trains, separated by long periods without bursts.

Cortical cells in dissociated culture form densely interconnected networks.

Within days after plating, neurons become electrically active, and soon after start to synchronize their activity into culture-wide bursts.

By growing cultures on multi-electrode arrays (Petri dishes with a grid of substrate-embedded electrodes), their electrical activity can be recorded non-invasively.

I developed software, MEABench, for online visualization and analysis of multi-electrode data, and used it to follow the development of cultures obtained from (E18) embryonic rats. Google(); req('single_work'); $('.js-splash-single-step-signup-download-button').one('click', function(e){ req_and_ready('single_work', function() ); new c. The end of my years as a graduate student only weeks away, I am reflecting on how many different people have contributed to the completion of my thesis.The Wagenaar Lab is happy to welcome our newest member, postdoctoral scholar Pegah Kassraian-Fard.Annette Stowasser’s paper quantifying visual processing in the leech has appeared in the Journal of Experimental Biology. The Wagenaar Lab is happy to welcome our newest member, postdoctoral scholar Sébastien Hayoz.In contrast to earlier published reports, I found that none of them induced changes in burst patterns or responses to test pulses that exceeded spontaneously occurring changes.We use cookies to make interactions with our website easy and meaningful, to better understand the use of our services, and to tailor advertising.A position is also available for a highly qualified and motivated graduate student. The detailed protocol for the experiments in Yusuke Tomina’s recent e Life paper appeared today in Bio Protocol.(The full text and videos can be downloaded without creating an account from my Publications page.) Drew Lehmkuhl and Arun Muthusamy’s joint first-author paper on neuronal responses to sensory stimuli from water waves appeared in the Journal of Experimental Biology. Funding for the lab is provided by the National Institute of Neurological Disorders and Stroke through grant R01NS094403 and by the California Institute of Technology.The Wagenaar Lab is part of the Division of Biology and Biological Engineering at the California Institute of Technology.We study the neuronal basis of sensory processing and sensory-guided behavior, with a specific focus on crossmodal sensory integration in the leech and on functional and anatomical circuit mapping.

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