Biosurfactant Production + Thesis

Biosurfactant Production + Thesis-2
This superbug was earlier engineered by Anand Mohan Chakrabarty (Indian-borne American) in 1979.The bug which was able to grow rapidly and produce surface-active substances that degrade hydrocarbon was a hybrid of Pseudomonas putida.

This superbug was earlier engineered by Anand Mohan Chakrabarty (Indian-borne American) in 1979.The bug which was able to grow rapidly and produce surface-active substances that degrade hydrocarbon was a hybrid of Pseudomonas putida.

Hydrocarbon-degrading bacterial populations are generally dominated by a few main genera, namely: Pseudomonas, Bacillus, Sphingomonas, Klebsiella and Actinobacteria in soils and sediments, and Pseudoalteromonas, Halomonas, Alcanivorax, and Acinetobacter in marine ecosystems (Bodour et al. It is not surprising therefore that a lot of biosurfactant or bioemulsifier producers belong to these same genera.

An estimate of the frequency of biosurfactant-producing strains within a microbial population cannot be easily determined, as it depends on the experimental procedures used.

The biosurfactant screening techniques employed were emulsification assay, emulsification index (E ), lipase activity, haemolytic assay, oil spreading, and tilted glass slide.

The bacterial isolate was identified based on phenotypic, biochemical, and molecular means.

Phospholipids are known to form major components of microbial membranes.

Wiącek (2012) was the first study that explored the effects of both electrolyte ions and ethanol molecules on 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) hydrolysis by phospholipase.The different classes of biosurfactant find application in different industrial processes.The attention given to the production of biosurfactants in recent times is mainly due to their potential utilization in food processing, pharmacology, cosmetics, oil exploration and exploitation industries, environmental management, and agriculture (Makkar and Cameotra 2002; Mulligan 2005).In addition, the biosurfactant showed emulsifying activity against the following hydrocarbons: petrol, kerosene, xylene, toluene, and diesel.The optimum cultural conditions (temperature, p H, carbon, nitrogen, hydrocarbon, inoculum concentration, and incubation time) for growth and biosurfactant production by K. The biosurfactant was characterized as a phospholipid using TLC, while the GC–MS analysis identified the phospholipid as phosphatidylethanolamine. pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil to produce biosurfactant and the effectiveness of the produced biosurfactant in emulsifying different hydrocarbons.For each soil source, soil samples were randomly collected from different points at depths between 0 and 15 cm using a hand-held soil auger and then bulked to get a composite sample.The samples were transported aseptically in sterile polythene bags to the laboratory for the analysis.Thin-layer chromatography (TLC) and gas chromatography mass spectrometry (GC–MS) analyses were used in the classification and characterization of the biosurfactant produced.The biosurfactant produced was applied on selected hydrocarbons to determine its emulsifying capacity.Biosurfactant from Pseudomonas aeruginosa SB30 was used in the EXXON Valdex oil spill in Alaska with 1 % being enough to remove two times the oil on water at temperatures of 40 °C and 80 °C.In 1990, a superbug (oil eating bug) was invented in the oil spill clean-up of the state of Texas in the USA.

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